PHYTOCHEMICAL TEST RAZAK MELADAPAHIT CAPSULE 250mg.

NOTIS KELULUSAN PENDAFTARAN PRODUK
PBKD 182 Pada 6/29/2006
BIRO PENGAWALAN FARMASEUTIKAL KEBANGSAAN
Pendaftaran Baru Tarikh : 7/6/2006

NO. RUJUKAN PRODUK: 2005070544902
NO. PENDAFTARAN : MAL06061476TC
NAMA PRODUK : Razak Meladapahit Capsule 250mg
NAMA & ALAMAT PENGELUAR : Razak Meladapahit [ MA0067950-W ]
:Lot 872, KG SRI GEMENCHEH,73200 GEMENCHEH, TAMPIN,N.SEMBILAN D.K.

REPORT OF PHYTOCHEMICAL TEST.
INSTITUT PENYELIDIKAN,
PERHUTANAN MALAYSIA.

Date of sample : 28 February 2008.
Sample : Melada pahit - 100/08.

Objective of phytochemical test ;
To detect the presence of some important phytochemical constituents that usually exhibit biological activities surch as alkaloids, sppnins, flavonoids, tannins, triterpenes and steroids.

# THE FOLLOWING TEST WERE CARRIEDS OUT ON 5.0g OF POWDER MATERIAL:

1. TEST FOR ALKALOIDS.
The plant was macerated in chloroform by followed by addition of ammoniacal chloroform. The extract was then treated with 10% sulphuric acid and tested with Mayer's reagent. Formation of white precipitates the presence of alkaloids.
Result:
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SAMPLE : 100/08
RESULT : -
CONCLUSION : Alkaloids were not detected
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2. TEST FOR SAPONINS.
The methanol extract was mixed with distilled water in a test tube. The formation of stable froth for at least 15 minutes indicates the presence of saponins.
Result:
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SAMPLE : 100/08
RESULT : 1+
CONCLUSION : Saponins were detected
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3. TEST FOR FLAVONOIDS
The chlorom extract was dissolved in ether and shaken in 10% ammonia solution. Formation of yellow colour in ammonia layer indicates the presence of flavonoids.
Result :
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SAMPLE : 100/08
RESULT : 2+
CONCLUSION : Flavonoids were detected
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4. TEST FOR TANNINS AND POLYPHENOLIC COMPOUNDS
The methanolic extract of plant material was mixed with 1% ferric chloride solution. Formation of blue black colour indicates the presence of hydrolysable tannins, while brownish-green indicates that of condensed tannins.
Result :
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SAMPLE : 100/08
RESULT : 1+
CONCLUSION : Condensed tannins were detected
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5. TEST FOR TRITERPENES/STEROIDS
The chloroform extract of plant material was tested using Liebermann-Buchard reagent. Formation of reddish colour indicates the presence of triterpenes and greenish colour for steroids.
Result :
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SAMPLE : 100/08
RESULT : -
CONCLUSION : Triterpenes/Steroids were not detected
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Prepared by :
Nuziah Hashim
Assistant Research officer
Chemistry unit
Medical Plant Programme

Date : 29th February 2008.

Verified by:
Dr. Ling Sui Kiong
Research officer
Chemistry unit
Medical Plant Programme